Under the microscope: Seeing cells grow. by harshameghadri

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· @harshameghadri · (edited)
$28.59
Under the microscope: Seeing cells grow.
<h1>Introduction</h1>

As a molecular biologist in the day; I work with a world that is quite complex and dynamic. It usually consists of one single Hero: The Cell. It is hard for me to imagine a day without thinking about the cell and its inner workings. The complex interactions that occur in a controlled manner to promote cellular and organismal growth keeps me curious and wanting to pursue research. 

Since the time Antonie Philips van Leeuwenhoek invented the microscope humans have pursued the search in the microscopic realms. It was unicellular microbes at first but over 300 years of innovation we are able to see more and understand better and answer the enigmatic questions. Human's thirst for knowing more has enabled better resolution of cells and their inner workings. This resolution has, in turn,  let us discover subcellular structures, localize proteins and visualize intra and extracellular signalling.

![Kras u251_BANNER.jpg](https://steemitimages.com/DQmfRCft6SVowr9KQ7NoehTZVYdKFFEwbAFCG1EY8qhMP6g/Kras%20u251_BANNER.jpg)

<br>

<center>This quote by the founder of micorscopy summarises </center>

>My work, which I've done for a long time, was not pursued in order to gain the praise I now enjoy, but chiefly from a craving after knowledge, which I notice resides in me more than in most other men. And therewithal, whenever I found out anything remarkable, I have thought it my duty to put down my discovery on paper, so that all ingenious people might be informed thereof.  - — Antonie van Leeuwenhoek, in a letter dated 12 June 1716 [Source: Wikipedia](https://en.wikipedia.org/wiki/Antonie_van_Leeuwenhoek).

</br>

<h2>Work: The love for Images meet Science</h2>

I have worked in few labs over the years as a Research student and have come to admire the diversity of cell types and how  the transformed cells function. Whenever I use the microscope, I feel baffled and very satisfied with what i see. It is very transformative indeed for me.



<h3>Cells at work</h3>

In this short blog post, I will show you all Dear Steemians some Images of various cells undergoing division or in the process of doing the same. Few images have signal activation on the cytoplasm when we induced localization of kRAS. All the cells exhibit beautiful morphological characteristics and the neural cells OR HeLa cells which are derived from cervical cancers.

<br>
<center>Cells were treated with an inhibitor of kRAS signalling so that we see kRAS localization in​ the cytoplasm _bright green spots_.</center>
![14C2.jpg](https://steemitimages.com/DQmQuodXPxC8mHScZdYDDm4sp3UGmG7fciyqcA4qsBZ23fC/14C2.jpg)

<center> HeLa cells were stained for nuclei(blue) and another protein (green)to visualize the localization</center>
![control siRNA 1_1.jpg](https://steemitimages.com/DQmNhvGdMfSpsiwF2RUcahvMniPYYntU1ZFnnPE7o7CaQr7/control%20siRNA%201_1.jpg)

<center>MeWo cells firing mitotic signalling cascades and undergoing division.</center>
![5C2.jpg](https://steemitimages.com/DQmdsCSvHxTWMcGK4FWz1x7HLsuHrV8X1iRnk6HPfcevB2T/5C2.jpg)

<center>U251 cells* under activated kRAS signalling</center>
![11C2_1.jpg](https://steemitimages.com/DQmRxpCHPX9xtXuWogeuySGqpyZPBqLRU6Axd843N64aVph/11C2_1.jpg)

<center>Few U251 cells are close to each other promoting intracellular signalling</center>
![2C2.jpg](https://steemitimages.com/DQmZWaNsfiDuDnP76RKwULDoAQcuhc2pANqtjghpkjTbF9Q/2C2.jpg)


<center>The cell with its nucleus stained with DAPI a dye that is nucleus specific.</center>
![Kras u251_1.jpg](https://steemitimages.com/DQmSXnY9DvE7L7tv5oJUwktyu9DDymh9UPfKtqSTW1DL436/Kras%20u251_1.jpg)

---------

It is a wonderful world seeing down through the microscope. I am happy to perform some experiments on a daily basis, Usually my time with microscopy is more exciting as it includes another favourite hobby; Photography.

I wanted to share snippets of the images. Just to try out blogging in STEM
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@appreciator ·
<p>This post has received gratitude of 17.92 % from @appreciator thanks to: @harshameghadri.</p>
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@qurator ·
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@harshameghadri ·
@Originalworks
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@originalworks ·
originalworks
The @OriginalWorks BETA V2 bot has upvoted(0.5%) and checked this post!
Some similarity seems to be present here:
http://www.ucmp.berkeley.edu/history/leeuwenhoek.html
This is an early BETA version. If you cited this source, then ignore this message! Reply if you feel this is an error.
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@harshameghadri ·
The source has been cited and hyperlinked​.
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@kriptonoob ·
Wow really love it... Like some space aliens creatures ;)
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@harshameghadri ·
Thank you @kriptonoob; you are welcome to follow my blog for more stuff like this in future.
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@kriptonoob ·
I just did that... I found you at AdSactly Discord ;)
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@bleury ·
Wowww I knew that you are a traveller! Haha
It's a very very nice posting. It's been 20 years since I've heard about 'the cell' the word itself. Lol
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@harshameghadri ·
Hahaha, Thank you YiYu. Well you have heard it again, hopefully not for the last time :P
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@bleury ·
Sure, I believe that you will post about cells again. :p
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vote details (11)
@kyrios ·
Wow I remember when my interest in science first began during my childhood days, I would go through my brother's biology books to read about a lot of things.  It's been a long time since I got  see the word "cell" again.
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@harshameghadri ·
Hahaha, true lot of my memories in school revolve around science classes esp biology. I am glad that you share the same interest. I hope in future I will write about cell often :) ; So follow my blog.

Cheers!!!!!
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@ackhoo ·
Thank you for sharing a little of your world... Fascinating, and brings me back to my biology class in school! :)
👍  
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